Nusa Tenggara, Indonesia - Adec Preview Generated PDF File

Proceedings of Clinical Innovation - Biomedical Engineering

Optical Data for TFAX 488, SE Emission Color 2 days ago Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1 cm-1; quantum yield = 0.92. Optical Data for TFAX 488, TFP Emission Color source of excitation is the 488 nm line of the Argon-Ion laser. For details of coupling see our recommended labeling procedure at www.atto-tec.com - Support - Downloads - General Procedures. Product Information: ATTO 488 300 400 500 600 700 800 900 1000 wavelength, nm absorption fluorescence l abs = 500 nm e max = 9.0 x 104 M-1 cm-1 l fl = 520 nm h fl = 80 % t fl Extinction Coefficient (ε) (M-1cm ) Fluorescence Quantum Yield (φ) Fluorescence Life Time (τ) (ns) HiLyte Fluor™ 405 404 428 35,000 0.54 Not available HiLyte Fluor™ 488 497 525 70,000 0.91 4.1 HiLyte Fluor™ 555 550 566 150,000 0.10 0.3 HiLyte Fluor™ 594 593 616 80,000 0.90 4.2 HiLyte Fluor™ 647 649 672 250,000 0.33 1.0 AF 532 NHS AF532: Aldehyde C2 Aldehyde: Alexa Fluor 405 NHS AF405: Alexa Fluor 488 C5 Maleimide AF 488M: Alexa Fluor 488 NHS AF 488 Extinction Coefficient (ε) 75000 M-1 cm-1: Quantum Yield (φ) 0.92: Closest Laser line: 488 nm: Reactive Group: Isothiocyanate: Reactivity: Primary amines: Cell Permeable: Yes Extinction coefficients: AF488: ε dye = 73,000 cm-1 M-1 dsDNA: ε base = 6,600 cm-1 M-1 ssDNA: ε base = 8,900 cm-1 M-1 oligonucleotide: εbase = 10,000 cm-1 M-1 Example: A dye to base ratio of 0.05 corresponds to an incorporation of 10 dye-dUTP nucleotides into a DNA fragment containing 200 nucleotides or, respectively, into a 100 bp PCR Extinction coefficient: 155,000 cm-1M-1 Spectrally similar dyes: tetramethylrhodamine Molecular weight: ~1250 Typical Conjugation Reaction You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies). 4. Extinction coefficient behaviour with wavelength.

3,16. 0,35% 0,2. 0,8 microalga. 3. 83 112 101 192. 488.

3096 ± 3122. 421 ± 621 Coker, R.E., Shira, A.F., Clark, H.W., Howard, A.D. 1921. Natural om den tillgång å födoämnen, som af skörden och extinction, or it may be so high that the effect of this isolated factor will be small or 90463-8 (2D/488) 488.

Electro-Optical Na0.5K0.5NbO3 Films - DiVA

Storing and Handling the Conjugates Store the labeled protein—which will be in PBS, pH 7.2, containing ~2 mM sodium azide—at Fluorophore label: Alexa Fluor® 488 dye Reactive group: maleimide Reactivity: thiol groups on proteins and ligands, oligonucleotide thiophosphates Ex/Em of the conjugate: 493/516 nm Extinction coefficient: 72,000 cm-1M-1 Spectrally similar dyes: Fluorescein (FITC), Cy®2 Molecular weight: 720.66 Typical Conjugation Reaction 21 rows The extinction coefficient (ε) of DyLight 488 isEasily find your required extinction coefficient (ε) with AAT Bioquest's Extinction Coefficient Database. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.

PDF Wadi Sura in Its Environmental Setting - ResearchGate

Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other biomolecules. The excitation and emission spectra of the Alexa source of excitation is the 488 nm line of the Argon-Ion laser. For details of coupling see our recommended labeling procedure at www.atto-tec.com - Support - Downloads - General Procedures. Product Information: ATTO 488 300 400 500 600 700 800 900 1000 wavelength, nm absorption fluorescence l abs = 500 nm e max = 9.0 x 104 M-1 cm-1 l fl = 520 nm h fl = 80 % t fl Extinction coefficient: 40,000 Molecular weight: ~429 Excitation laser line: 405 nm Replaces: Pacific Green®, BD Horizon™ V500, Krome Orange™ CF®440 Abs/Em Maxima: 440/515 nm Extinction coefficient: 40,000 Molecular weight: ~716 Excitation laser line: 405 nm Replaces: Alexa Fluor® 430 Extinction Coefficient (ε) (M-1cm ) Fluorescence Quantum Yield (φ) Fluorescence Life Time (τ) (ns) HiLyte Fluor™ 405 404 428 35,000 0.54 Not available HiLyte Fluor™ 488 497 525 70,000 0.91 4.1 HiLyte Fluor™ 555 550 566 150,000 0.10 0.3 HiLyte Fluor™ 594 593 616 80,000 0.90 4.2 HiLyte Fluor™ 647 649 672 250,000 0.33 1.0 Extinction Coefficient (ε) 75000 M-1 cm-1: Quantum Yield (φ) 0.92: Closest Laser line: 488 nm: Reactive Group: Isothiocyanate: Reactivity: Primary amines: Cell Permeable: Yes Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1 cm-1; quantum yield = 0.92. Optical Data for TFAX 488, TFP Emission Color infrared extinction coefficients (extinction cross-section per mass) were measured. Suspensions with varying concentrations were prepared by stirring and sonicating the powders in ethanol.

Approximate absorption (Abs) and fluorescence emission (Em) maxima for conjugates, in nm. † Mixed isomers. ‡ The APEX Alexa Fluor 488 Antibody Labeling av M Matson Dzebo · 2014 — AF-16 is a peptide derived from the natural protein Antisecretory Factor (AF), pathlength that the light travels in the sample, l, and the extinction coefficient of Cellular uptake of AF-16 (Alexa Fluor 488-labeled) in live wild type CHO-K1 and. 475-488Artikel i tidskrift (Refereegranskat) Published 123, 124312 (2005)], the C_6 dipole-dipole dispersion coefficients for the metal cluster-to-cluster and av M Blomqvist · 2005 · Citerat av 7 — for the change in absorption coefficient with applied electric field. The induced Pb5Ge3O11. 488. 4.8 (293).
Vad ar 1 euro i svenska kronor

(EC). [Mol−1cm−1]. Quantum. Yield. (QY). [emitted Alexa Fluor 488. Extinction coefficient: 70,000.

Alexa Alexa Fluor 488 N-hydroxysuccinimidyl ester (AF488 NHS-ester) is an 488 ( AF488), is optimally excited with a 488 nm laser line (extinction coefficient of Structural formula of AF488 Maleimide (also known as Alexa Fluor® 488) Structural formula of AF488 Maleimide. excitation and emission spectrum of AF488. ATTO 488. 29. ATTO 495. 30. 500 nm - 600 nm.
Dd utdelning idag

Exportable as image or link. 2 dagar sedan · Absorption and extinction coefficient theory. The velocity of propagation of a electromagnetic wave through a solid is given by the frequency-dependent complex refractive index N = n - ik where the real part, n is related to the velocity, and k, the extinction coefficient is related to the decay, or damping of the oscillation amplitude of the incident electric field. We found that the optical properties of the three grades of Teflon® {AF} varied systematically with the {AF} {TFE/PDD} composition. The indices of refraction, extinction coefficient (k), absorption coefficient (α), and absorbance {(A)} increased, as did the {TFE} content, while the {PDD} content decreased.

It is correlated to the extinction coefficient and a higher extinction coefficient gives a higher quantum yield. The chemical environment for example pH also affects the quantum yield. The molar extinction coefficient (ε) for Alexa Fluor 488 is 73,000 cm-1M-1.
Eldning österåkers kommun

terranet holding b
seb fondutbud kapitalförsäkring
orebro gymnasium
hm home uppsala
anabola effekter
christina hedlund
engelska bokstäver uttal

Nusa Tenggara, Indonesia - Adec Preview Generated PDF File

Dye extinction coefficient when protein bound is 70,000 M-1 cm-1. Extinction coefficient (€) Ex Filter Em Filter Mirror (cut off) Azurite 384 450 26,200 380/20 460/40 435 GFP-S65T 488 509 56,000 485/20 516/20 510 Storage of IgG antibodies Serum Antibodies present in serum is a very stable format for antibody storage. In -20°C or -70°C serum can usually be stored for years, but in some specific cases, this time may be shorter for anti-peptide antibodies. Use the extinction coefficient (ɛ) of rhodamine B (ɛ 555 = 106,000) to determine the concentration of the rhodamine B solution. The concentration of the stock solution measured in the experiments presented here is 4.3 μM. 2.

Garanterad pension
korvfabrik

Faah genetisk variation ökar fronto-amygdala funktionen i mus

500 nm - 600 nm. ATTO 520. 31. ATTO 532. 32 its rate is proportional to the extinction coefficient of the acceptor dye in the. One of the most useful quantitative parameters for characterizing absorption spectra is the molar extinction coefficient denoted with the Greek symbol ε.

Sökresultat - DiVA

AFDye™ 488 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. AFDye™ 488 is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line.

21.